Fig. 5.

Effects of cellular cholesterol sequestration on porcine nidovirus propagation at early time points postinfection. PAM-pCD163 and Vero cells were pretreated with MβCD and were mock infected or infected with PRRSV (A) and PEDV (B), respectively. At 48 hpi, the virus-infected cells were fixed, and virus infectivity was determined by measuring the percentage of cells expressing N proteins of PRRSV or PEDV by FACS. The data are presented as the mean values of three independent experiments, and error bars represent standard deviations. *, P = 0.001 to 0.05; **, P < 0.001