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Expression of HCoV-OC43 and influenza A proteins in HEK-293 cells. HEK-293 cells were transfected with the expression vector pAcGFP1 coding for HCoV-OC43 ns2a, ns5a, M or N proteins, or for influenza A NS1 protein. Three days following transfection, the expression of viral proteins was confirmed by indirect immunofluorescence assay using anti-GFP monoclonal antibody
