Table 1.

Virus RNA shedding determined by qRT-PCR in the feces of gnotobiotic calves orally inoculated with PDCoV (OH-FD22) or PEDV (PC21A) during acute to mid-stages of viral infection

Calf #		Calf age when inoculated (day)	Inoculum titer (log10 GE/calf)	Viral titers (log10 GE/ml of rectal swab fluid or fecal sample)										Onset of fecal virus shedding (PID)	PID when virus titer peaked
				Post-inoculation day (PID)
				0	1	2	3	4	5	6	7	8	9
PDCoV-inoculated	1a	6	9.6	<4.6b	<4.6	8.1c	8.4	7.8	7.9	7.7	7.8	7.8	6.6	2	3
	2	3	9.6	<4.6	<4.6	7.2	5.7 (EUd)							2	2
	3	7	9.0	<4.6	<4.6	<4.6	6.0	8.0	NDe	ND	6.6	6.1	5.5 (EU)	3	4-6
	4	4	9.0	<4.6	<4.6	<4.6	8.8	7.9	ND	ND	8.1	8.4	8.7 (EU)	3	3 (ND)
PEDV-inoculated	5a	4	12.2	<4.8b	<4.8	<4.8	<4.8	<4.8	<4.8	<4.8	<4.8	<4.8	<4.8	.	.
	6	4	10.2	<4.8	<4.8	<4.8	<4.8	<4.8	<4.8	<4.8	<4.8	EU		.	.
	7	5	12.5	<4.8	<4.8	<4.8	<4.8	<4.8	<4.8	<4.8	<4.8	EU		.	.
Negative control	8	3f	.	<4.6/<4.8b	<4.6/<4.8	<4.6/<4.8	EU							.	.

^aVirus shedding of calves #1 and #5 monitored long-term

^bReal-time PCR-negative; <4.6 and <4.8 log₁₀ GE/ml for PDCoV and PEDV, respectively (detection limit of the qRT-PCR for rectal swab fluid)

^cReal-time PCR-positive; log₁₀ viral titer (GE/ml of rectal swab fluid)

^dEU; euthanized

^eND; not determined or not available

^fAt euthanasia