Table 1.
Nucleotide sequences of the oligonucleotides used for PCR amplification and sequencing
| Primer name† | Nucleotide sequence (5′ → 3′) | Nucleotide position* |
|---|---|---|
| Oligo d(T)-anchor | GACCACGCGTATCGATGTCGAC(T)16 | - |
| Cakobu-1F | GTAACTAAGTGTGTGCCCAATC | 110-132 |
| Cakobu-1R | GCATGCAGTGGCGTCACTAGTTG | 360-382 |
| Cakobu-2R | TTCCTCATCGCAGTGGCAATAA | 871-892 |
| Cakobu-2F | TTATTGCCACTGCGATGAGGAA | 871-892 |
| Cakobu-3R | TCAGTGGTGACGTTGTTGCCATT | 1253-1275 |
| Cakobu-3F | TGTCACCAACATCTACGGCAAT | 1234-1255 |
| Cakobu-4R | GAGCCGTTGACGGTGACCTGCA | 2018-2039 |
| Cakobu-4F | TGCAGGTCACCGTCAACGGCT | 2018-2038 |
| Cakobu-5R | CCACTGTCTTCCAGTAGGACT | 2837-2857 |
| Cakobu-5F | AGTCCTACTGGAAGACAGTGG | 2847-2867 |
| Cakobu-6R | ATGAAGAAGTTGGAGAGCATCT | 3450-3471 |
| Cakobu-6F | AGATGCTCTCCAACTTCTTCAT | 3450-3471 |
| Cakobu-7R | TTGGTGTCAGCGTCAGCGGTCA | 4221-4242 |
| Cakobu-7F | TGACCGCTGACGCTGACACCAA | 4221-4242 |
| Cakobu-8R | TATCGTGACGAGTTCACGACAG | 4929-4950 |
| Cakobu-8F | CTGTCGTGAACTCGTCACGATA | 4939-4960 |
| Cakobu-9R | TGGAGTCCTCACGCTGTTGCCA | 5954-5975 |
| Cakobu-9F | TGGCAACAGCGTGAGGACTCC | 5954-5974 |
| Cakobu-10R | GGTTGATGTTGACACCGGGTT | 6659-6679 |
| Cakobu-10F | AACCCGGTGTCAACATCAACC | 6669-6689 |
| Cakobu-11R | TAATCCAGATCATACACCTGAGA | 7325-7347 |
| Cakobu-11F | TCCTCGGTGGTCTTATTGACTA | 7211-7231 |
| Cakobu-12R | AAGAACAGTTAGAAAAG | 8275-8291 |
| Oligo(dT)15 | TTTTTTTTTTTTTTT | - |
† F, forward; R, reverse
*Nucleotide positions for CaKoV correspond to the submitted sequence under GenBank accession no. JN088541