Fig. 3.

G2/M arrest promotes HHV-6A replication. A Asynchronously growing HSB-2 cells were treated with DMSO or 0.5 μg/ml nocodazole for 24 h, and the cell cycle profiles were then analyzed by flow cytometric analysis. The 2N (diploid) and 4N (Tetraploid) DNA contents represent the G1 and G2/M phases of the cell cycles, respectively. B and C Synchronously growing HSB-2 cells and asynchronously growing control cells were infected with HHV-6A at an MOI of 10, followed by incubation for 72 h. The amount of viral U22 gene was determined by real-time PCR (B). Viral protein accumulation was analyzed by western blot with anti-HHV-6 gp60/110 antibody, and the expression level was quantitatively analyzed and compared to β-actin expression using a densitometer (C). The data shown are the mean ± SD from three independent experiments. **P < 0.01 compared with the control