Table 1.

Primers for the multiplex and routine PCRs

Test	5’-3’ Sequence	Position	Reference or source	Amplicon size (bp)
CPV-F CPV-R	ATCACAGCAAACTCAAGCAGACTT AAATGGTGGTAAGCCCAATGCTC	2695-2718a 3384-3362a	This study	690
CCoV-F CCoV-R	ACATGGTATATCTATGTGCGCAA TGCAAGGCGCACTTGAGAT	18902-18924b 19153-19135b	This study	252
CAV-F CAV-R	TGTGCCCATCGACAAGGAA CTAATAGAAGCGGCCCAACTG	10454-10472c 10886-10866c	This study	433
CPV1 CPV2	GAAGAGTGGTTGTAAATAATT CCTATATAACCAAAGTTAGTAC	3017-3037d 3698-3677d	[13]	682
CCoV1 CCoV2	TCCAGATATGTAATGTTCGG TCTGTTGAGTAATCACCAGCT	6729-6748e 7138-7118e	[12]	212
CCoV3	GGTGTCACTCTAACATTGCTT	6927-6947e
CAV1 CAV2	CGCGCTGAACATTACTACCTTGTC CCTAGAGCACTTCGTGTCCGCTT	CAVI: 769-790f CAVII: 1387-1408g CAVI: 1273-1253f CAVII: 2413-2392g	[6]	508 (CAVI) 1030 (CAVII)

^aOligonucleotide position in reference to the canine parvovirus 2a strain CPV/CN/SD6/2014 sequences (GenBank accession no. KR002801.1)

^bOligonucleotide position in reference to the canine coronavirus strain K378 sequence (GenBank accession no. KC175340.1)

^cOligonucleotide position in reference to the canine adenovirus sequence (GenBank accession no. NC_001734.1)

^dOligonucleotide position in reference to the CPV-2 strain CPV-b sequence (GenBank accession no. JQ268283.1)

^eOligonucleotide position in reference to the CCoV strain Insavc-1 sequence (GenBank accession no. D13096.1)

^fOligonucleotide position in reference to the canine adenovirus type 1 E3 region sequence (GenBank accession no. S38238.1)

^gOligonucleotide position in reference to the Cav-2 strain Toronto A26-61 sequence (GenBank accession no. U77082.1)

^gOligonucleotide position in reference to the sequence of the Cav-2 strain Toronto A26-61 (GenBank accession no. U77082.1)