Table 1.
Comparison of the FTD multiplex RT-PCR assay with monoplex real-time RT-PCR as the reference. The sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and kappa value are shown
| Pathogen | No. of samples (monoplex RT-PCR/FTD assay) | FTD PCR performance | |||||||
|---|---|---|---|---|---|---|---|---|---|
| +/+ | +/− | −/+ | −/− | Sensitivity (%) | Specificity (%) | PPV (%) | NPV (%) | Kappa | |
| Adenovirus | 9 | 2 | 5 | 353 | 81.8 | 98.6 | 64.3 | 99.4 | 0.71 |
| Human bocavirus | 19 | 7 | 11 | 332 | 73.1 | 96.8 | 63.3 | 97.9 | 0.65 |
| Coronavirus NL63 | 0 | 1 | 1 | 367 | na | 99.7 | na | 99.7 | na |
| Coronavirus OC43 | 5 | 6 | 1 | 357 | 45.5 | 99.7 | 83.3 | 98.3 | 0.58 |
| Coronavirus 229E | 5 | 2 | 2 | 360 | 71.4 | 99.4 | 71.4 | 99.4 | 0.71 |
| Enterovirus/parechovirus1 | 3 | 1 | 4 | 361 | 75 | 98.9 | 42.9 | 99.7 | 0.54 |
| Human metapneumovirus | 38 | 2 | 8 | 321 | 95 | 97.6 | 82.6 | 99.4 | 0.87 |
| Influenza virus A, universal2 | 8 | 2 | 2 | 357 | 80 | 99.4 | 80 | 99.4 | 0.79 |
| Influenza virus B | 0 | 0 | 0 | 369 | na | 100 | na | 100 | na |
| Parainfluenza virus 1 | 4 | 4 | 0 | 361 | 50 | 100 | 100 | 98.9 | 0.66 |
| Parainfluenza virus 2 | 1 | 3 | 1 | 364 | 25 | 99.7 | 50 | 99.2 | 0.33 |
| Parainfluenza virus 3 | 6 | 1 | 1 | 361 | 85.7 | 99.7 | 85.7 | 99.7 | 0.85 |
| Parainfluenza virus 4 | 1 | 0 | 1 | 367 | 100 | 99.7 | 50 | 100 | 0.67 |
| Respiratory syncytial virus | 65 | 5 | 3 | 296 | 92.9 | 99 | 95.6 | 98.3 | 0.93 |
| Rhinovirus | 30 | 1 | 13 | 325 | 96.8 | 96.2 | 69.8 | 99.7 | 0.79 |
na: not applicable
1FTD assay does not distinguish between enterovirus and parechovirus
2FTD assay does not subtype influenza virus A