Fig. 2.

Geometric mean antibody titers for GIII.2 BoNoV and nebovirus in the feedlot and veal calves from different regions of the US during 1999-2001. Three groups of 6- to 7-month-old feedlot calves from New Mexico (NM) (A), Arkansas (AR) (B), and Ohio (OH) (C) and a group of 7- to 10-day-old Ohio veal calves (D), comprising animals commingled from different regions of the US, were studied. Serum samples were collected pre-arrival or on arrival at the farms for the NM, AR and OH calves, and 35 days after arrival for all groups to monitor seroprevalence and seropositive increase rates during the period. The VLPs of Bo/CV186-OH/00/US and Bo/NB/80/US strains were expressed using the baculovirus expression system and were used in ELISA to measure IgG antibodies