Table 1.

Species-specific primers and probes used for coronavirus detection in multiplex and monoplex PCR assays

Target	Orientation	Primer or probe sequence (5’ → 3’)	Concentration
HCoV-229E	S	CAG TCA AAT GGG CTG ATG CA	300 nM
	AS	CAA AGG GCT ATA AAG AGA ATA AGG TAT TCT	300 nM
		FAM-CCC TGA CGA CCA CGT TGT GGT TCA-TAMRA	100 nM
HCoV-NL63	S	GCG TGT TCC TAC CAG AGA GGA	50 nM
	AS	GCT GTG GAA AAC CTT TGG CA	300 nM
		FAM-ATG TTA TTC AGT GCT TTG GTC CTC GTG AT-TAMRA	100 nM
HCoV-OC43	S	CGA TGA GGC TAT TCC GAC TAG GT	900 nM
	AS	CCT TCC TGA GCC TTC AAT ATA GTA ACC	900 nM
		FAM-TCC GCC TGG CAC GGT ACT CCC T-TAMRA	125 nM