Table 2.
Detection of human coronaviruses using the CT12-mCODEHOP one-step RT-PCR assay in nasopharyngeal swabs that were positive for coronavirus by real-time PCRa
| Coronavirus | Nb | Detected | Not detected | ||
|---|---|---|---|---|---|
| N (%) | Ct IQM (IQR)c | N (%) | Ct IQM (IQR)c | ||
| HCoV-229E | 16 | 1 (6.7 %) | 21.14 | 15 (93.7 %) | 28.28 (22.79-31.87) |
| HCoV-NL63 | 17 | 9 (53 %) | 23.63 (22.0-27.52) | 8 (47 %) | 28.88 (27.10-34.28) |
| HCoV-OC43 | 36 | 19 (52.8 %) | 26.21 (22.44-27.56) | 17 (47.2 %) | 33.04 (30.68-34.85) |
| All HCoVs | 69 | 29 (42.0 %) | 25.32 (22.03-27.52) | 40 (58.0 %) | 30.68 (27.75-34.51) |
aHCoVs were initially detected using a multiplex coronavirus real-time RT-PCR and then typed by monoplex real-time PCR assays. Cycle threshold number (Ct) values obtained by both methods were similar, and the monoplex-based results are presented in the table
bTotal number of HCoV-positives
cIQM, interquartile median and IQR, interquartile range, for Ct values