Summary
Plaque assay in DBT cells with DEAE-dextran and trypsin presents a titration system for MHV3 as sensitive as the LD50 assay in mice.
Keywords: Cell Culture, Hepatitis, Infectious Disease, Trypsin, Virus Type
Footnotes
With 1 Figure
References
- 1.Appleyard G., Maber H. B. Plaque formation by influenza viruses in the presence of trypsin. J. gen. Virol. 1974;25:351–357. doi: 10.1099/0022-1317-25-3-351. [DOI] [PubMed] [Google Scholar]
- 2.Bradburne A. F., Tyrrell D. A. J. The propagation of “Coronaviruses” in tissue-culture. Arch. ges. Virusforsch. 1969;28:133–150. doi: 10.1007/BF01249379. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 3.Haff R. F. Plaque formation by a mouse hepatitis virus. Virology. 1962;18:507–508. doi: 10.1016/0042-6822(62)90049-1. [DOI] [PubMed] [Google Scholar]
- 4.Hirano N., Fujiwara K., Hino S., Matumoto M. Replication and plaque formation of mouse hepatitis virus (MHV-2) in mouse cell line DBT culture. Arch. ges. Virusforsch. 1974;44:298–302. doi: 10.1007/BF01240618. [DOI] [PubMed] [Google Scholar]
- 5.Mallucci L. Observations on the growth of mouse hepatitis virus (MHV-3) in mouse macrophages. Virology. 1965;25:30–37. doi: 10.1016/0042-6822(65)90248-5. [DOI] [PubMed] [Google Scholar]
- 6.Tobita K., Kilbourne E. D. Genetic recombination for antigenic markers of antigenically different strains of influenza B virus. J. Virol. 1974;13:347–352. doi: 10.1128/jvi.13.2.347-352.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 7.Tyrrell D. A. J., Almeida J. D., Cunningham C. H., Dowdle W. R., Hofstad M. S., McIntosh K., Tajima M., Ya Zakstelskaya L., Easterday B. C., Kapikian A., Bingham R. W. Coronaviridae. Intervirology. 1975;5:76–82. doi: 10.1159/000149883. [DOI] [PMC free article] [PubMed] [Google Scholar]