Table 3.

Detection of PDCoV-specific IgA, IgG and neutralizing antibody titers in the sera of Gn pigs inoculated with original and tissue-culture-grown PDCoV OH-FD22

Pig no.	Inoculation virus	PID* 1			PID 7			PID 14			PID 23/24
		IgA	IgG	VN*	IgA	IgG	VN	IgA	IgG	VN	IgA	IgG	VN
2	OH-FD22-P5	<4	<4	<4	<4	<4	64	16	16	256	256	1024	1024
4	OH-FD22-P20	<4	<4	<4	<4	<4	64	16	16	256	256	256	1024
6	OH-FD22-P40	<4	<4	<4	<4	<4	256	64	64	1024	1024	1024	4096
2#	OH-FD22 (original)	<4	<4	<4	<4	<4	64	64	256	256	256	1024	1024
8	-	<4	<4	<4	<4	<4	<4	<4	<4	<4	<4	<4	<4

* PID, postinoculation days; VN, virus neutralization

^#See reference [12]. Pig 2 was inoculated with the original PDCoV OH-FD22 as reported

Note: PDCoV-specific IgG and IgA antibodies in serum were determined by indirect ELISA using cell culture PDCoV OH-FD22 as the coating antigen. The neutralizing antibody titers were tested by the 80 % plaque reduction neutralization test (PRNT₈₀). PRNT₈₀ was determined as the reciprocal of the highest serum dilution that neutralized 80 % of the plaques as compared to that in the virus-only control wells. The IgA, IgG and VN antibody titers of hyperimmune PDCoV serum OH-DC97 (positive control serum) were 1024, 4096 and 4096, respectively