Fig. 4.

Gene silencing of Atg5 or Beclin-1 by siRNA inhibits NDV production. U251 cells were transfected with 100 pmol siRNA duplexes targeted to either Atg5 or Beclin-1 mRNA, or control siRNA, for 72 h. (A) The relative expression of Atg5 and Beclin-1 proteins was examined by immunoblot analysis with specific antibody to Atg5 or Beclin-1, respectively. (B) Transfected cells were infected with NDV (MOI = 0.01) and the virus yield was determined at different intervals. Data are presented as the mean ± SD for triplicate assays. **, p < 0.01