Fig. 1.

miR-142a-3p is upregulated during PHEV infection in vitro and in vivo. (a) PHEV-infected N2a cells were collected at different time points. The expression of miR-142a-3p was measured by qRT-PCR. (b) miR-142a-3p expression in mouse brain tissues after infection with PHEV was measured by qRT-PCR. (c) Cells that were pre-treated as indicated above were analyzed by qRT-PCR to measure the level of PHEV mRNA. (d) PHEV mRNA expression in mouse brain tissues after infection with PHEV was measured by qRT-PCR. (e) N2a cells were transfected with miR-142a-3p mimic (100 nM), inhibitor (400 nM), or NC (100 nM). Expression of miR-142a-3p was then measured by qRT-PCR, with the housekeeping gene U6 used as an internal control for normalization. (f) N2a cells were transfected with the miR-142a-3p mimic, inhibitor, or NC for 12 h, followed by PHEV infection for 24 h. Lysates were harvested and tested by qRT-PCR. (g) Cells were pre-treated as indicated above, lysed, and analyzed using a Western blotting assay to determine the level of PHEV N protein. Data were normalized to β-actin. All of the data are representative of at least three independent experiments (*, p < 0.05; **, p < 0.01)