Fig. 1.

Optimization of RPA reaction time. CPV-2a genomic DNA was amplified with RPA for different lengths of time, and a clear DNA band with the expected size (~210 bp) could be visualized by agarose gel electrophoresis after a 10-min reaction. Semi-quantification by measuring the DNA band density revealed that the DNA yield doubled when the reaction was done for 20 min, but no significant difference was observed in the product yields of 20-min, 30-min and 40-min reactions. M, DNA marker; lanes 1–4, DNA products from reactions incubated for 10 min, 20 min, 30 min and 40 min, respectively