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. 2016 Jan 5;161(4):1015–1018. doi: 10.1007/s00705-015-2738-y

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© Springer-Verlag Wien 2016

This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.

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Fig. 2 — Sensitivity of RPA. Different amounts of CPV-2a genomic DNA were amplified by RPA (panel A) and conventional PCR (panel B). The detection limit for RPA was 10 copies/reaction. In contrast, the detection limit for conventional PCR was 100 copies/reaction. M, DNA marker; lane 1, 10⁵ copies; lane 2, 10⁴ copies; lane 3, 10³ copies; lane 4, 10² copies; lane 5, 10¹ copies; lane 6, 10⁰ copies. Five independent reactions were performed, and similar results were obtained