Fig. 1.

Multiple sequence alignment and phylogenetic analysis. A. Multiple amino acid sequence alignment of TSHSV-HP2, HP3 and HP4 with specific proteins of other viruses. The aligned sequences were as follows: 1ab protein (Chinese broad-headed pond turtle arterivirus; AVM87331.1); putative 1b protein (Pebjah virus; AKI29956.1); ORF1a polyprotein (porcine reproductive and respiratory syndrome virus; ADX07034.1); polyprotein (Kibale red colobus virus 2; AHH53949.1); ORF1ab polyprotein (equine arteritis virus; AAR14192.1); 1ab protein (Guangdong greater green snake arterivirus; AVM87321.1); ORF1b polyprotein, partial (porcine reproductive and respiratory syndrome virus; BAP16275.1); putative 1b protein (southwest baboon virus 1; YP_009067063.1). Identical (red) and similar (box) residues are indicated. B. Phylogenetic relationships between TSHSV, arteriviruses and coronaviruses based on the complete genome sequence. The tree was generated using the neighbor-joining method