Table 1.
Primer and probe sequences
| Serial no. | Gene | Primer/probe | Sequence 5’–3’ |
|---|---|---|---|
| 1 | NP | Forward (real- time) | 5’GCACTCAAAAGATACCCTAGRATRGAYATAC-3’ |
| 2 | NP | Reverse (real-time) | 5’-AATAAACTTTCTGCTTTGCTKCCTGTWGA-3’ |
| 3 | NP | Probe (Real time) | FAM-ATGAGCCTAAWGCTTTCCCATACTCAATGAA- BHQ1 |
| 4 | NP | T7HMPVNFP1 | TAATACGACTCACTATAGGGCATACAAACATGCTATATTAAAAGAGTCTC |
| 5 | NP | HMPVNFP2 | GAAGAAATAGACAAAGAAGCAAG |
| 6 | NP | HMPVNFP3 | AACAATATAATGCTAGGGCATGTATC |
| 7 | NP | HMPVNRP1 | ATTATTGGTGTGTCTGGTGCTGA |
| 8 | NP | HMPVNRP2 | CCAGATTCAGGACCCATTTCTC |
| 9 | NP | HMPVNRP3 | TTTTGCTGCTTCATTACCCATG |
| 10 | G | HMPVGAFP1 | GGGACAAGTGGCCATGGA |
| 11 | G | HMPVGAFP2 | AACAGCACTAAGTATGGCACT |
| 12 | G | HMPVGAFP3 | GCACAGCTGCAACCCAAAC |
| 13 | G | HMPVGARP1 | GGGTCTTCTTGCTTGGTTC |
| 14 | G | HMPVGARP2 | GATCGCTGCTTTGAGTTGTG |
| 15 | G | HMPVGBFP1 | GGGACAAGTAGTTATGGAGG |
| 16 | G | HMPVGBFP2 | CCAACTCAACAGTCCACAGAA |
| 17 | G | HMPVGBFP3 | AGCAGCACARGAAAAAGACC |
| 18 | G | HMPVGBRP1 | GGGTGRTGAGCTGGTGTG |
| 19 | G | HMPVGBRP2 | GTCTTTGTTCTGCTTKCACTTG |
| 20 | L | HMPVLRP | GGRAGATAGACATTRACAGTGG |