Fig. 2.

GW3965 affects NDV entry and replication. (A) GW3965 did not affect NDV attachment. DF-1 cells were incubated with NDV (MOI = 1) at 4 °C for 1 h in the presence of GW3965 (1 μM). The viral gRNA copy number was determined by qRT-PCR assay. (B) GW3965 inhibited NDV entry. DF-1 cells were first incubated with NDV (MOI = 1) at 4 °C for 1 h and were then cultured at 37 °C in the presence of GW3965 (1 μM) for 1 h. The cells were washed three times. The viral gRNA copy number was determined by qRT-PCR. (C) GW3965 inhibited NDV replication. After infected with NDV (MOI = 1), DF-1 cells were incubated with GW3965 (1 μM). Viral gRNA copy numbers were determined by qRT-PCR at 8 hpi and 10 hpi