Fig. 5.

NDV infection disturbs LXR and its downstream lipogenic gene expression. (A-B) The effect of the NDV infection on the expression of lipogenic genes. DF-1 cells were infected with NDV (MOI = 1). Total RNA was extracted at 6, 12, and 24 hpi. The mRNA levels of LXR-α, SREBP-1, PPAR-γ1, FASN, and ABCA1 were determined. (C-G) The effect of GW3965 on the expression of lipogenic genes in infected DF-1 cells. DF-1 cells were infected with NDV (MOI = 1) in the presence or absence of GW3695 (1 μM) for 12 h. The relative mRNA levels of LXR-α, SREBP-1, PPAR-γ1, FASN, and ABCA1 were determined by qRT-PCR. The RNA copy number in NDV-infected cells was considered to be 1