Fig. 3.

Assay of the virucidal activity of GRFT against JEV infection. (A) The virus was pretreated with different concentrations of GRFT for 1.5 h, and the mixture was diluted below the IC₅₀ of GRFT before inoculating the cells. The remaining infectivity was determined by plaque assay. (B) The cells were pretreated with GRFT for 1.5 hrs and washed three times with PBS before inoculated with the virus for 1.5 h. The plaque assay was performed, and the results were analyzed. The bars represent the means and SD