Fig. 4.

Evaluation of the effect of the MOI on the antiviral activity of GRFT in BHK-21 cells. Different concentrations of GRFT were incubated with virus at different MOIs for 1.5 h. BHK-21 cells were then incubated in the presence of the GRFT/JEV mixture for 1.5 h. The cells were washed before adding DMEM containing 2 % FCS and re-culturing. Plaque assay (A) and western blot analysis (B) were performed at 48 hpi