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Sequences of primers used for real-time PCR
| Gene | Primer sequence (5′–3′) |
|---|---|
| JEV (NS1) | Forward: ACACTCGTCAGATCACAGGTTCA |
| Reverse: GCCAGAAACATCACCAGAAGG | |
| β-Actin (BHK-21 cells) | Forward: CATCCGTAAAGACCTCTATGCCAAC |
| Reverse: ATGGAGCCACCGATCCACA |
NS1, JEV nonstructural gene 1
