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. 2020 Mar 20;40(3):BSR20200164. doi: 10.1042/BSR20200164

Figure 4. DHA inhibits GREM1 expression in human breast cancer cells.

Figure 4

Cells were starved overnight and stimulated with DHA (25 μM) for 24 h. RNA was collected and analyzed by qPCR analysis (A) and proteins were subjected to Western blot analysis (B). Cells were starved overnight and pretreated with TGF-β (10 ng/ml) for 24 h and then incubated with DHA (25 μM) for another 24 h. RNA was collected and analyzed by qPCR analysis (C) and proteins were subjected to Western blot analysis (D). **, P<0.01 and ***, P<0.001.