Fig. 1.
Nonspecific effects of the proteasome inhibitor MG132 on luciferase activity. Treatment with MG132 after 24 h (A) and 48 h (B) resulted in dramatic reduction of luciferase activity in subgenomic HEV replicon (p6-Luc) (mean ± SD, n = 12), HCV replicon (Huh7-ET) (mean ± SD, n = 4) and Huh7 cells constantly expressing a control luciferase gene under the control of the PGK promoter (Huh7-PGK) (mean ± SD, n = 4). MG132 treatment did not strongly affect cellular metabolic activity or viability, as determined by MTT assay (OD490 value) (mean ± SD, n = 4), although a minor inhibitory effect was observed after treatment at 10 µM for 48 h