Extended Data Figure 2:

CLCF1 secreted by cancer-associated fibroblasts and LUAD cell lines. (a) Quantitative real-time PCR (qRT-PCR) of CLCF1 mRNA expression in human paired cancer-associated fibroblasts (CAFs) relative to normal lung fibroblasts (NLFs) from the same patient. qRT-PCR data are representative of n = 3 independent experiments. GAPDH and HPRT expression were used for normalization. *** P < 0.001 using two-tailed unpaired Student’s t-test. Data represented as mean ± S.D. (b) Representative cropped western blot analysis of conditioned media CLCF1 levels of indicated LUAD cell lines (representative of n = 2 independent experiments). (c) ELISA results showing CLCF1 levels (pg/mL) in conditioned media from indicated cell lines (n = 3 independent experiments). Data represented as mean ± S.D.