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. 2020 Mar 20;8:e8830. doi: 10.7717/peerj.8830

Figure 3. Effects of ICA on cell cycle-related factors expression in PDGF-BB-stimulated RPE cells.

Figure 3

The mRNA levels of p53, p21, CDK4, CDK6 and cyclin D1 were determined by qPCR (A), and the protein levels of p53, p21, CDK4, CDK6 and cyclin D1 were detected by western blot assay (B and C). Control: blank control group without PDGF-BB; PDGF-BB: 20 ng/ml PDGF-BB; PDGF-BB + ICA: 20 ng/ml PDGF-BB + 40 µM ICA. Data are expressed as mean ± SD (n = 3). #P < 0.05, ##P < 0.01, ###P < 0.001 vs. control group; *P < 0.05, **P < 0.01, ***P < 0.001 vs. PDGF-BB group.