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. 2007 Sep 11;54(2):245–252. doi: 10.1007/s10344-007-0137-8

Table 2.

Primers used in virus detection by PCR and RT-PCR (Erles et al. 2004; Desario et al. 2005)

Virus/primers Primer sequence (5′–3′) Nucleotide position Amplicon size
Canine Parainfluenza virus (CPIV)
CPI1 AGTTTGGGCAATTTTTCGTCC 120–140a 667 bp
CPI2 TGCAGGAGATATCTCGGGTTG 786–766a
CPI3 CGTGGAGAGATCAATGCCTATGC 521–543a 187 bp
CPI4 GCAGTCATGCACTTGCAAGTCACTA 702–678a
Canine Adenovirus 1 (CAV1) and 2 (CAV2)
CA 1,2 F CTGGGCGGGATTTAGAGGGTGG 18,804–18,825b 704 bp
CA 1,2 R CAAGGGCGTGGGCGGAGTTAGA 19,507–19,486b
Canine Parvovírus (CPV)
CP1 CAGGAAGATATCCAGAAGGA 4,003–4,022c 583 bp
CP2 GGTGCTAGTTGATATGTAATAAACA 4,561–4,585c

aGenBank accession number AF052755

bGenBank accession number U77082

cGenBank accession number AY42955