Table 3.
Comparison of monoplex PCR Ct-values in multiplex PCR-positive and -negative samples, respectively
| Pathogen | Median Ct-value for monoplex PCR | ||
|---|---|---|---|
| Multiplex PCR-positive samples (n) | Multiplex PCR-negative samples (n) | p-value* | |
| Adenovirus | 30 (9) | 38 (1) | na |
| Bocavirus | 25.5 (12) | 36 (9) | 0.0001 |
| Coronavirus HKU1 | 25 (3) | na (0) | na |
| Coronavirus NL63 | na (0) | 39 (1) | na |
| Coronavirus OC43 | 24 (1) | 39 (5) | na |
| Coronavirus 229E | 34 (4) | 39 (1) | na |
| Enterovirus | 36 (3) | na (0) | na |
| Human metapneumovirus | 33 (19) | 38.5 (6) | 0.031 |
| Influenza virus A, universal | 34 (6) | 39 (1) | na |
| Influenza virus A/H1N1 | na (0) | na (0) | na |
| Influenza virus A/H3N2 | na (0) | na (0) | na |
| Influenza virus B | na (0) | na (0) | na |
| Parainfluenza virus 1 | 29 (3) | 40 (3) | 0.1 |
| Parainfluenza virus 2 | 29 (3) | 36 (1) | na |
| Parainfluenza virus 3 | 28 (4) | 40 (2) | na |
| Parainfluenza virus 4 | na (0) | 32 (1) | na |
| Respiratory syncytial virus A | 28 (15) | 22 (1) | na |
| Respiratory syncytial virus B | 27 (27) | 36 (9) | 0.002 |
| Rhinovirus | 36.5 (30) | 35.5 (12) | 0.82 |
| Bordetella pertussis | na (0) | na (0) | na |
| Chlamydia pneumoniae | 36 (1) | 39 (3) | na |
| Legionella pneumophila | na (0) | 39 (2) | na |
| Mycoplasma pneumoniae | 36.5 (7) | 37 (4) | 0.9 |
*Mann–Whitney U-test; na: not applicable