Fig. 6.

Secretion of cytokines in immunized groups with various formulations: The levels of IFN-γ (a), IL-5 (b), IL-10 (c) secretion were determined in the supernatants using ELISA as mean absorbance at 450 nm ± SD for each sample. All analyses were performed in duplicate for each sample; Granzyme B secretion measured by ELISA (d): All analyses were performed in triplicate for each sample. The results represent mean values calculated from triplicate samples as well as the standard deviation (SD) as error bars. *p < 0.05; **p < 0.01, ***p < 0.001, ns non-significant (p > 0.05)