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Visualization of cytoskeletal filaments in synoviocytes. After fixation and permeabilization, synoviocytes were labeled with antibodies to either vimentin or a-tubulin followed by Alexa-conjugated secondary antibodies. For visualization of actin filaments, the permeabilized cells were labeled with Alexa 488-conjugated phalloidin. Each cytoskeletal system has its characteristic organization in synoviocytes
