Table 1.

Nucleotide sequences of the primers and probes used for multiplex Q-PCR and sequencing

Multiplex Q-PCR primers and probes	Sequence
hBoV NP-1 gene (88 bp)a
Forward primer	5′-AGAGGCTCGGGCTCATATCA-3′ (2478)c
Reverse primer	5′-TCTTCATCACTTGGTCTGAGGTCTT-3′ (2541)c
Probe	5′-VIC-AGGAACACCCAATCARCCACCTATCGTCT-TAMRA-3′ (2500)c
AdV hexon gene (132 bp)b
Forward primer	5′-GCCACGGTGGGGTTTCTAAACTT-3′ (21)e
Reverse primer	5′-GCCCCAGTGGTCTTACATGCACATC-3′ (152)e
Probe	5′-FAM-TGCACCAGACCCGGGCTCAGGTACTCCGA-TAMRA-3′ (89)e
Sequencing primers	Sequence
hBoV NP-1 gene (313 bp)a
Forward primer	5′-CTCCAGCAAGTCCTCCAAAC-3′ (2253)c
Reverse primer	idem hBoV Q-PCR
hBoV VP2 gene (965 bp) a
Forward primer	5′-CAGCTGGAGGCAATGCTAC-3′ (4059)d
Reverse primer	5′-ATGACGTGGGCTGGATGTAT-3′ (5005)d
Forward primer semi-nested	5′-AATTGCAAACCCATCACTCTCA-3′ (4558)d
Reverse primer semi-nested	5′-CCCAGACTTGATTTGGAAACATC-3′ (4645)d

hBoV = human bocavirus; AdV = adenovirus

^aDesigned as part of the present study

^bHeim et al. [12], adapted according to the sequence information available in GenBank

^{c, d, e}The location of the primers and probes is indicated in parentheses (the reference sequence is DQ000495^(c) and DQ000496^(d) for hBoV and AB330083^(e) for AdV)