Fig. 3.

Applied methods for modified base detection using Nanopore sequencing. a Detection of open chromatin regions using DNA methyltransferase accessibility. DNA in open chromatin regions is specifically methylated. Open chromatin regions are detected as highly methylated regions by Nanopore sequencing. b Detection of DNA replications using base analogs. The replicated regions are labeled by pulse labeling during S phase. The replicated regions are detected as labeled regions by Nanopore sequencing. c Detection of nascent RNA using base analogs. Nascent RNAs are labeled by pulse labeling using base analogs. Nascent and preexisting RNAs are classified by the existence of base analogs