Fig. 6.

Efficiencies of module performance. Top left: swab sample extracts spiked with 4x10⁶ PFU of bacteriophage-fr were lysed, RNA/DNA isolated and quantified via RT-qPCR and qPCR against bacteriophage-fr and human targets. Top right: 10³ copies RSVB in vitro transcript were amplified in a nested multiplex RT-PCR, once conventionally in a reaction tube (pos. ctrl.) and once on-chip (on-chip RT-PCR), while the negative control (neg. ctrl.) was amplified in a reaction tube without in vitro transcripts. Bottom left: Influence of blocking reagents on the nested multiplex RT-PCR on-chip. Reactions were carried out on-chip without the use of blocking reagents (LoC-V1), use of blocking reagents in RT-PCR 1 and PCR 2 (LoC-V2) or use of blocking reagents only in RT-PCR 1 (LoC-V3). Positive control was done conventionally in reaction tubes without blocking reagents. Bottom right: DNA obtained by ResPlex II multiplex RT-PCR was hybridised and labelled for LiquiChip detection either conventionally in a reaction tube (Reference) or using dried reagents on–chip (LoC). (MFI = Median fluorescence intensities values measured by QIAGEN LiquiChip 200 workstation). (Per graph one representative experiment is shown.)