Table 3.
Comparison of 16S rDNA sequencing, MALD-TOF MS and MIDI MIS identification analysis of 103 bacterial isolates collected during the three periods in Norway, Sweden and Finland
Methods | No. of isolates classified (%) | No. of isolates non-classified (%) |
---|---|---|
16S r DNA sequencing | 85 (83) | 18 (17) |
MALDI-TOF | 81 (79) | 22 (21) |
MIDI | 77 (75) | 26 (25) |
The isolates were classified to order or higher. The 16S rDNA sequences were taxonomically identified by comparison with reference databases, the RDP database and the BLASTn database in NCBI Genbank. Only isolates identified using all three methods (N = 103) are included in this comparison