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Robustness testing
| Parameter | SDa | RSD (%)a |
|---|---|---|
| Mobile phase composition | 0.0073 | 0.96 |
| Amount of mobile phase | 0.0054 | 0.65 |
| Time from spotting to chromatography | 0.0049 | 0.58 |
| Time from chromatography to scanning | 0.0054 | 0.65 |
a Average of three concentrations 1, 3 and 5 μg spot−1
