Fig. 3.

Inhibition of the ATPase activity of SaPriA. The ATPase activity of purified SaPriA was analyzed using the vanadate-sensitive colorimetric assay. The ATPase activity assay (2 mL of reaction volume) for SaPriA (2 μM) was analyzed in 20 mM HEPES (pH 7.0), 5 mM of MgCl₂, 1 mM of ATP, and 35 μM of flavonoid for 2 h, and then the OD₆₁₀ was analyzed. The reaction mixture with (lane 1) or without SaPriA (lane 2) was analyzed. The reaction mixture with SaPriA was analyzed in the presence of kaempferol (lane 3), myricetin (lane 4), dihydromyricetin (lane 5), galangin (lane 6), quercetin (lane 7), and myricitrin (lane 8)