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. 2020 Jan 6;99(4):456–462. doi: 10.1177/0022034519898144

Figure 2.

Figure 2.

Human serum immunoreactivity against lysates from citrullination-competent and citrullination-incompetent Porphyromonas gingivalis does not differ. (A) Preabsorbed serum anticitrullinated protein antibodies (ACPA) titration was performed by preabsorbing sera from ACPA+, ACPA– rheumatoid arthritis (RA) patients, and healthy control (HC) sera on microtiter plates coated with whole cell lysates from P. gingivalis W50, PG1424, or 10% bovine serum albumin (BSA; negative control), followed by detection of remaining serum ACPA with anti-CCP ELISA. Results are expressed as mean ± SEM. **P ≤ 0.01. ***P ≤ 0.001. One-way analysis of variance, followed by Sidak’s post hoc test. (B–E) Assessment of human serum immunoreactivity against specific components in lysates from citrullinated-competent and citrullinated-deficient P. gingivalis. Representative immunoblots are shown of (B) whole cell lysates and (D) supernatants from P. gingivalis W50 (W) and PG1424 (P) probed with serum from ACPA+ and ACPA– RA patients and healthy controls and horseradish peroxidase–conjugated anti-human IgG antibody. Arrow in panel B indicates band used for analysis in panel F. (C, E) Corresponding densitometry analysis of band intensity on immunoblots. Results in scatter-dot plots are expressed as mean ± SEM and unpaired student’s t test comparing W50 and PG1424 in each serum group. (F) Densitometry analysis of 11-kDa band (arrow in B) intensity on immunoblots of W50 or PG1424 whole cell lysates incubated with ACPA+ RA patient serum. Results are presented as mean ± SEM and unpaired Student’s t test.