Table 1.
The molecular masses of peptide fragments produced by digesting PE-KAA-2 and PE-ESA-2 with trypsin and their corresponding sequence in ESA-2
| PE-KAA-2 | PE-ESA-2 | Sequence in ESA-2 | |||
|---|---|---|---|---|---|
| Peak no. | Determined molecular mass | Peak no. | Determined molecular mass | Calculated molecular mass | |
| 1 | 1023.6 | 1 | 1023.6 | 1024.1 | 230HNQNITAVK238 |
| 2 | 1275.4 | 2 | 1275.6 | 1276.3 | 247NLDGTCTYER256 |
| 3 | 1069.9 | 3 | 1069.5 | 1070.2 | 180TLEGTMQYK188 |
| 4 | 1171.4 | 4 | 1170.8 | 1172.3 | 113TLTGTMTYER122 |
| 5 | 746.2 | 5 | 746.3 | 746.9 | 123EGPIGFK129/257EGPIGFK263 |
| 6 | 831.3 | 6 | 831.3 | 831.9 | 189GEGPIGFR196 |
| 7 | 1588.7 | 7 | 1588.7 | 1589.7 | 96SGQGVLAVNITSSDGGK112 |
| 8 | 2539.5 | 8 | 2540.7 | 2540.6 | 130GTQSGGDTYNVENQWGGSSAPWNK153 |
| 9 | 2540.3 | 9 | 2539.5 | 2540.6 | 130GTQSGGDTYNVENQWGGSSAPWNK153 |
| 10 | 2550.8 | 10 | ND | ||
| 11 | 2364.7 | –a | |||
| 12 | 1001.5 | – | |||
| – | 13 | 3403.5 | 3402.7 | 28GNQNVMAVDVNSSDGGANLNGTMTYSGEGPIGFK61 | |
| 14 | 957.4 | 14 | 957.4 | 958.1 | 154AGIWALGDR162 |
| 15 | 3490.5 | – | |||
| 16 | 3450.1 ± 3.96b | – | |||
| 17 | 3348.2 | 17 | 3348.5 | 3348.6 | 65RGESNVYDVENQWGGSSAPWHAGGQFVIGSR95 |
| 18 | 3347.3 | 18 | 3347.5 | 3348.6 | 65RGESNVYDVENQWGGSSAPWHAGGQFVIGSR95 |
| 19 | 3375.3 | 19 | 3375.9 | ||
| – | 20 | 3293.9 | 3293.5 | 199LSGANNYSVENQWGGSSAPWNAAGDWLIGDR229 | |
| – | 21 | ND | |||
| 22 | ND | 22 | 3293.0 | 3293.5 | 199LSGANNYSVENQWGGSSAPWNAAGDWLIGDR229 |
| 23 | 2759.8 ± 10.99b | – | |||
| 24 | 2763.3 | 24 | 2763.9 | 2764.0 | 3YTVQNQWGGSSAPWNDAGLWILGSR27 |
| 25 | 2763.2 | 25 | 2763.1 | 2764.0 | 3YTVQNQWGGSSAPWNDAGLWILGSR27 |
| 26 | 2800.2 | 26 | 2796.2 | 2764.0 | 3YTVQNQWGGSSAPWNDAGLWILGSR27 |
ND not determined
aNo consistent peak
bStandard error of >1.0 are represented