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. 2015 Dec 12;18(2):215–231. doi: 10.1007/s10126-015-9684-2

Fig. 4.

Fig. 4

The predicted amino acid sequence of native KAA-2. The results of sequence prediction by peptide mapping in KAA-2 are shown using ESA-2 sequence as a template. The peptide fragments produced by trypsin digestion of PE-KAA-2 are represented with arrows. The peak numbers examined by Edman degradation and their determined sequences are underlined. The sequences whose peptide masses were consistent with those of ESA-2 are represented in bold. Unidentified amino acids in KAA-2 are represented as lowercase letter of corresponding sequence in ESA-2. Shaded regions in the sequence were used to design the degenerated primers for cDNA cloning