Table 2.
Hemagglutination-inhibition test of native and recombinant KAAs with sugar compounds
| Sugars and glycoproteins | Minimum inhibition concentration | |||
|---|---|---|---|---|
| KAA-1 | KAA-2 | His-rKAA-1 | rKAA-1 | |
| Monosaccharides and disaccharide (mM)a | >100 | >100 | >100 | >100 |
| Glycoproteins (mg/ml) | ||||
| N-glycan type | ||||
| High-mannose type | ||||
| Yeast mannan | 31.3 | 62.5 | 31.3 | 62.5 |
| High-mannose and complex types | ||||
| Porcine thyroglobulin (PTG) | 15.6 | <7.8 | 15.6 | 31.3 |
| Asialo-PTG | <7.8 | <7.8 | <7.8 | 15.6 |
| Complex type | ||||
| Transferin | >1000 | >1000 | 1000 | >1000 |
| Asialo-transferin | 500 | 250 | 250 | 500 |
| N/O-glycan type | ||||
| Fetuin | >1000 | >1000 | >1000 | >1000 |
| Asialo-fetuin | 250 | 250 | 250 | 500 |
| O-glycan | ||||
| Bovine submaxirally mucin (BSM) | 62.5 | 125 | 125 | 250 |
| Asialo-BSM | 31.3 | 31.3 | 62.5 | 125 |
aMonosaccharides (100 mM) (Glc, Man, Gal, GlcNAc, GalNAc, Rha, Fuc, Xyl, NeuAc) and a disaccharide (lactose) did not inhibit hemagglutination activities of four lectins