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Summary of purification of the isolectins from E. denticulatum. Fresh alga (1 kg) was used as a starting material
| Extraction and purification step | Protein (mg) | Total activity (× 103 HUa mL−1) | Specific activity (× 103 HU mg−1) | Yield (%) |
|---|---|---|---|---|
| Extraction | 614.4 | 865.3 | 1.4 | 100.0 |
| Ethanol precipitation | 85.1 | 654.6 | 7.7 | 75.6 |
| Gel filtration | ||||
| EDAs | 33.2 | 510.7 | 15.4 | 59.0 |
| Ion exchange | ||||
| EDA-1 | 6.9 | 115.0 | 16.7 | 13.3 |
| EDA-2 | 13.4 | 248.2 | 18.5 | 28.7 |
| EDA-3 | 4.2 | 78.3 | 18.8 | 9.1 |
aHemagglutination units (hemagglutination titer)
