Table 3.
Hemagglutination-inhibition test of the three isolectins isolated from E. denticulatum. The hemagglutination-inhibition test was carried out as described in the Experimental
| Sugar and glycoprotein | EDA-1 | EDA-2 | EDA-3 |
|---|---|---|---|
| Sugar (mM) | |||
| Monosaccharidesa | –b | – | – |
| Lactose | – | – | – |
| Fucoidan | – | – | – |
| Glycoprotein (μg mL−1) | |||
| Transferrin | – | – | – |
| Fetuin | 62.5 | 62.5 | 62.5 |
| Porcine thyroglobulin | 1.9 | 1.9 | 1.9 |
| Asialo-porcine thyroglobulin | 1.9 | 1.9 | 1.9 |
| Bovine thyroglobulin | 7.8 | 7.8 | 7.8 |
| Asialo-bovine thyroglobulin | 7.8 | 7.8 | 7.8 |
| Yeast mannan | 3.9 | 3.9 | 3.9 |
| Bovine submaxillary mucin | 31.2 | 31.2 | 31.2 |
| Asialo-bovine submaxillary mucin | 31.2 | 31.2 | 31.2 |
| Porcine stomach mucin | – | – | – |
Values indicate the lowest concentration of sugar (mM) and glycoprotein (μg mL−1) at which complete inhibition of hemagglutination (titer 4) was achieved
aThe monosaccharides examined are described in Experimental
bIndicates no inhibition at 100 mM for monosaccharides and at 2000 μg mL−1 for glycoproteins