Table 4.
Oligosaccharide-binding activity of EDA-2
Oligosaccharides | Binding activity (%) | |||
---|---|---|---|---|
EDA-2 | ESA-2a | KSA-2b | KAA-2c | |
Complex type | ||||
1 | 0.0 | 0.0 | 10.3 | 0.0 |
2 | 0.0 | 0.0 | 0.0 | 0.0 |
High-mannose type | ||||
3 | 55.1 | 79.0 | 42.5 | 95.8 |
4 | 100.0 | 91.5 | 100.0 | 100.0 |
5 | 40.1 | 68.5 | 56.6 | 17.2 |
6 | 100.0 | 93.4 | 100.0 | 100.0 |
7 | 44.5 | 66.4 | 46.7 | 34.6 |
8 | 98.7 | 84.9 | 94.6 | 76.7 |
9 | 43.3 | 69.9 | 51.4 | 65.0 |
High-mannose type | ||||
10 | 54.1 | 68.2 | 53.7 | 49.1 |
11 | 98.6 | 88.2 | 100.0 | 74.2 |
12 | 21.4 | 52.8 | 28.4 | 61.5 |
13 | 37.1 | 79.0 | 37.1 | ND |
14 | 0.0 | 0.0 | 0.0 | ND |
Oligomannose | ||||
15 | 0.0 | 0.0 | 0.0 | 0.0 |
16 | 0.0 | 0.0 | 0.0 | 0.0 |
Core pentasaccharide | ||||
17 | 0.0 | 0.0 | 0.0 | 0.0 |
Binding activity was determined by the centrifugal ultrafiltration-HPLC method as described in the Experimental and expressed as a ratio (%) of the amount of a bound oligosaccharide to that of the added oligosaccharide. The structures of PA-oligosaccharides are presented in Fig. 8
ND not determined
aCited from Hori et al. (2007)
bCited from Hung et al. (2011)
cCited from Sato et al. (2011a)