Fig. 5.
Chloroquine suppresses autophagic protein degradation, not interferon pathways. a Cells were treated with 10−5 M of chloroquine (CQ) or 100 U/ml of IFNα for 24–48 h. Phosphorylation of PKR was assessed by western blot analysis. GAPDH was used as loading control. b Ultrastructural analysis showing the effect of chloroquine on the number of autolysosomes. Huh-7/Rep-Feo cells were incubated with chloroquine for 18 h. Autolysosomes were identified as the double membrane vesicles (arrow heads) of cytoplasm in Huh-7 Rep/Feo. The number of autolysosomes in 100 μm2 of cytoplasm was counted by using transmission electron microscopy. Data represent mean ± SEM of individual preparations from pictures (*P < 0.05 vs. control by ANOVA). c Western blot analysis of LC3 in Huh7 Rep/Feo. The lysate of Huh7 Rep/Feo treated with chloroquine for 4–8 h were immunoblotted with LC3. GAPDH was used as loading control