Fig. 1.

Downregulation of Beclin1 and activation of the PI3K/AKT signaling pathway in reinfection pneumonia. a Determination of Beclin1 expression in all included samples by RT-qPCR, *p < 0.05 vs. the normal group. b Determination of PI3K/AKT signaling pathway-related proteins in 15 samples by western blot analysis, *p < 0.05 vs. the normal group. The measurement data were expressed in the form of mean ± standard deviation, and the comparison among multiple groups should be based on one-way analysis of variance, followed by Dunnett’s post hoc test. The experiment was repeated three times. Normal: n = 15, Pne: n = 15, Flu + Pne: n = 45. RT-qPCR, reverse transcription quantitative polymerase chain reaction.