Fig. 2.

Successful establishment of mouse model of influenza virus/S. pneumoniae infection. a Survival analysis of mice in each group, *p < 0.05 vs. the Flu + SP group. b The detection of the amount of virus in the lungs of the mice, *p < 0.05 vs. the 1st day. c Detection of Streptococcus pneumoniae in bronchoalveolar lavage fluid of mice, *p < 0.05 vs. the 1st day. d Measurement of MPO activity in bronchoalveolar lavage fluid of mice, *p < 0.05 vs. the control group. e Observation of pathological changes of lung tissue in mice by HE staining (× 400). The measurement data were expressed in the form of mean ± standard deviation, and the comparison among multiple groups should be based on one-way analysis of variance, followed by Dunnett’s post hoc test. The experiment was repeated 3 times. N = 10. SP, Streptococcus pneumoniae; MPO, myeloperoxidase; HE, hematoxylin and eosin.