Fig. 2.

Quantitative real-time RT-PCR for MxA in healthy children. Expression of MxA for three different genotypes of C-123A and G-88T was measured in RNA from 45 healthy children (20 from the northern population and 25 from the southern population) stimulated with IFNβ1a. Normalization for mRNA quantity was performed with human GAPDH control primers for each sample and final abundance figures adjusted to yield an arbitrary value of 1 for -123CC or -88GG genotype. Columns mean from triplicate measurements; the vertical bars indicate the standard deviation (SD). a Correlation of MxA mRNA expression with C-123A genotypes in the northern population. Compared to the CC carriers, the A allele carriers had a markedly elevated MxA transcription (P < 0.05; t test). b Correlation of MxA mRNA expression with G-88T genotypes in the northern population. Compared to the GG carriers, the T allele carriers had a markedly elevated MxA transcription (P < 0.05; t test). c Correlation of MxA mRNA expression with MxA haplotypes in the northern population. The subjects that are homozygous for the major C-123A and G-88T haplotype (CC/GG) had lower levels of MxA mRNA than subjects that are heterozygous (CA/GT) (t test; P < 0.05). d Correlation of MxA mRNA expression with C-123A genotypes in the southern population. Compared to the CC carriers, the A allele carriers had a markedly elevated MxA transcription (P < 0.05; t test). e Correlation of MxA mRNA expression with G-88T genotypes in the southern population. Compared to the GG carriers, the T allele carriers had a markedly elevated MxA transcription (P < 0.05; t test). f Correlation of MxA mRNA expression with MxA haplotypes in the southern population. The subjects that are homozygous for the major C-123A and G-88T haplotype (CC/GG) had lower levels of MxA mRNA than subjects that are heterozygous (CA/GT) (t test; P < 0.05)