Skip to main content
. Author manuscript; available in PMC: 2021 Mar 3.
Published in final edited form as: Cell Metab. 2020 Mar 3;31(3):642–653.e6. doi: 10.1016/j.cmet.2020.02.003

Figure 1.

Figure 1.

Design of the ultrasensitive H2O2 indicator. (A) Neighbor joining-based phylogenetic tree of the OxyR-RD domain sequence based on the multiple sequence alignment derived from Supplementary Figure 1. The tested 11 OxyR regulatory domain sequences are highlighted in bold, and the regulatory domain sequence of HyPer7 (from Neisseria meningitidis) is highlighted in red. (B) cpYFP from HyPer probe was integrated into 11 selected OxyR-RD protein domains. The most sensitive version was further improved by mutagenesis.