KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Bacterial and Virus Strains | ||
| Escherichia coli, M15 strain | Qiagen | N/A |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Isopropyl β-D-1-thiogalactopyranoside (IPTG) | Fisher Scientific | BP1755-10; CAS: 367-93-1 |
| Dithiothreitol (DTT) | Roche | 10708984001; CAS: 3483-12-3 |
| Leupeptin | Carl Roth | CN33.3; CAS: 103476-89-7 |
| 4-(2-aminoethyl)benzenesulfonyl fluoride hydrochloride (AESBF) | Carl Roth | 2931.3; CAS: 34284-75-8 |
| Benzamidine hydrochloride hydrate | Sigma-Aldrich | B6506; CAS: 206752-36-5 |
| DNAse I | Sigma-Aldrich | DN25; CAS: 9003-98-9 |
| Ni2+-Sepharose 6 Fast Flow | GE Healthcare | 17531802 |
| 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid (HEPES) | Sigma-Aldrich | H3375; CAS number: 7365-45-9 |
| Imidazole | Merck | 1.04716.1000; CAS: 288-32-4 |
| Tris(2-carboxyethyl)phosphine (TCEP) | Roth | HN951; CAS: 51805-45-9 |
| Diethylenetriaminepentaacetic acid (DTPA) | Sigma-Aldrich | 285560; CAS: 67-43-6 |
| Hydrogen peroxide (H2O2) | Sigma-Aldrich | 95321; CAS: 124-43-6 |
| tert-butyl hydroperoxide | Sigma-Aldrich | B2633; CAS Number 75-91-2 |
| cumene hydroperoxide | Sigma-Aldrich | 247502; CAS Number 80-15-9 |
| benzoyl hydroperoxide | Sigma-Aldrich | 228877; CAS Number 94-36- |
| 4-hydroperoxy-2-nonenal | Cayman Chemical | 10004413; CAS Number 7439-43-2 |
| Peroxynitrite (ONOO−) | Cayman Chemical | 81565; CAS: 14042-01-4 |
| Oxidized glutathione (GSSG) | Roche | 105635; CAS:103239-24-3 |
| Reduced glutathione (GSH) | Sigma-Aldrich | 64251; CAS: 70-18-8 |
| 6-(2-Hydroxy-1-methyl-2-nitrosohydrazino)-N-methyl-1-hexanamine (MAHMA NONOate) | Sigma-Aldrich | M1555; CAS: 146724-86-9 |
| Xanthine oxidase | Sigma-Aldrich | X4376; CAS: 9002-17-9 |
| Xanthine | Sigma-Aldrich | X0626; CAS: 69-89-6 |
| Catalase | Sigma-Aldrich | C9322; CAS: 9001-05-2 |
| D-alanine | Sigma-Aldrich | A7377 |
| Auranofin | Enzo | BML-EI206-0100 |
| Rotenone | Sigma-Aldrich | R8875 |
| PEG-catalase | Sigma-Aldrich | C4963 |
| PolyJet™ In Vitro DNA Transfection Reagent | Signagen Laboratories | SL100688 |
| Experimental Models: Cell Lines | ||
| NIH-3T3 fibroblasts | Collection of cell cultures of vertebrates, Institute of Cytology Russian Academy of Sciences, Russia, originally obtained from ATCC | RRID:CVCL_0594 ATCC CRL 1658 |
| HeLa-Kyoto | EMBL | RRID:CVCL_1922 |
| EA.hy926 | ATCC | CRL-2922 |
| K-562 | ATCC | CCL-243 |
| Experimental Models: Organisms/Strains | ||
| Zebrafish, Danio rerio, AB-Tu strain | ZFIN | https://zfin.org/action/genotype/view/ZDB-GENO-010924-10 |
| Oligonucleotides | ||
| 5’-attagctacaggatcccgggaattcgccaccatg-3’ Forward primer for HyPer7 cloning into PQE-30 | This paper | N/A |
| 5’-attggatcgaaagcttacagtcggcctcgagtca-3’ Reverse primer for HyPer7 cloning into PQE-30 | This paper | N/A |
| Recombinant DNA | ||
| pQE30-HyPer7 | This paper | N/A |
| pQE30-HyPer | (Belousov et al., 2006) | N/A |
| pQE30-HyPer3 | (Bilan et al. 2012); Addgene | https://www.addgene.org/42132/ |
| pC1-HyPerRed | Belousov lab, Addgene | https://www.addgene.org/48249/ |
| FusionRed-Lifeact-7 | Addgene | https://www.addgene.org/54778/RRID:Addgene_54778 |
| pC1-HyPer3-dmito | (Mishina et al., 2019) | N/A |
| pC1-HyPer2-IMS | (Ermakova et al.,2014) | N/A |
| pC1-HyPer2-NES | Belousov lab | pC1-HyPer2-NES |
| pC1-HyPer2-NES | Belousov lab | N/A |
| pC1-HyPer3 | Belousov lab, Addgene | https://www.addgene.org/42131/ |
| Software and Algorithms | ||
| Prism 7 | GraphPad Software | https://www.graphpad.com |
| FIJI | Fiji.sc | |
| MEGA software package | (Kumar et al., 2016) | https://www.megasoftware.net |
| MetaFluor® Imaging System | Molecular Devices,San Jose, California, United States | https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/metafluorfluorescence-ratioimagingsoftware.pdf |
| iTOL(Interactive Tree of Life) | (Letunic and Bork, 2016) | https://itol.embl.de |
| Jalview | (Waterhouse et al., 2009) | http://www.jalview.org |
| CytExpert Software v2.0 | Beckman Coulter, USA | https://www.beckman.com/flowcytometry/instruments/cytoflex/software |